Interaction of Light with Dye-Doped Calcium Phosphate Nanoparticles
Open Access
- Author:
- Russin, Timothy John
- Graduate Program:
- Physics
- Degree:
- Doctor of Philosophy
- Document Type:
- Dissertation
- Date of Defense:
- April 10, 2009
- Committee Members:
- Peter C Eklund, Dissertation Advisor/Co-Advisor
Peter C Eklund, Committee Chair/Co-Chair
Nitin Samarth, Committee Member
Vincent Henry Crespi, Committee Member
Joan Marie Redwing, Committee Member - Keywords:
- nanoparticles
indocyanine green
local field effect
bioimaging
penetration depth
quantum yield
quantum efficiency
fluorescence - Abstract:
- In this work we present work on a novel amorphous calcium phosphate nanoparticle system for use in bioimaging and drug delivery applications. The system, by virtue of its synthesis, can be made to encapsulate and protect any number of molecules that are not suitable for biological applications on their own; for example, medication that is poorly soluble in aqueous solution can be encapsulated for delivery, or fragile optical molecules can be encapsulated to protect them from the local environment. We have encapsulated the near-infrared dye indocyanine green, which has bene ficial properties for optical imaging (low biotoxicity, absorption and emission at a minimum of tissue absorption). There are two original works presented in this thesis. The fi rst describes the measurement of the quantum yield of the indocyanine green-doped nanoparticles, as well as the development of a theoretical method to extract the molecular quantum yield of a fluorophore encapsulated in a dielectric sphere from eff ective quantum yield measurements of nanoparticle dispersions in solution. The second work is an application of diff use scattering theory to the problem of light propagation in biological tissue; speci fically, the limits on penetration depth for photodynamic therapy and bioimaging.