Non-enzymatic Glucose Sensors based on Laser-induced Graphene integrated with Skin-interfaced Microfluidic component for On-body measurement
Restricted (Penn State Only)
Author:
Liu, Shangbin
Graduate Program:
Engineering Science and Mechanics (MS)
Degree:
Master of Science
Document Type:
Master Thesis
Date of Defense:
March 09, 2022
Committee Members:
Albert Segall, Program Head/Chair Larry Cheng, Thesis Advisor/Co-Advisor Jian Hsu, Committee Member
Keywords:
biosensor glucose mircofludic
Abstract:
The recent increased interest in wearable and mobile technologies has prompted increasing
research efforts in developing non-invasive glucose monitoring platforms. In comparison with
enzymatic glucose sensors, non-enzymatic glucose sensors have low cost, high stability, and easy
integration and have the potential to be wearable real-time monitoring sensors. However, due to
the trace amount of glucose in sweat, the sensitivity of existing non-enzymatic glucose sensors still
needs to be further improved. To demonstrate practical approaches with bimetals or 3D porous
structures, the porous laser-induced graphene (LIG) on flexible polymers demonstrates good
conductivity and a simple fabrication process to integrate sensing materials. The
uniform electroless plating of the nickel and gold layer on LIG electrodes demonstrates
significantly enhanced sensitivity and an extensive linear range for glucose sensing. The sensor
with the porous LIG foam exhibits a high sensitivity of 1080 μA·mM−1·cm−2
, whereas a further
increased sensitivity of 3500 μA·mM−1·cm−2
is obtained with LIG fibers (LIGF). Impressively, a
significant linear range (0–30 mM) can be achieved by changing the bias voltage from 0.5 to 0.1 V
due to the Au coating. In order to store and evaluate the sweat content of sweat on the skin, this
work integrates a porous encapsulated reaction chamber with an alkaline solution containing an
alkaline solution with a soft, skin-interface microfluidic component. In addition, we tested glucose
levels in human sweat and cell culture media before and after meals using the amperometric
response of the sensor that demonstrates non-enzymatic sweat glucose biosensors are expected to
be the prospect of commercial wearable devices.
