Dehydration stress and Mayaro virus vector competence in Aedes aegypti
Open Access
- Author:
- Manzano Alvarez, Jaime
- Graduate Program:
- Entomology
- Degree:
- Master of Science
- Document Type:
- Master Thesis
- Date of Defense:
- June 06, 2023
- Committee Members:
- Gary Felton, Program Head/Chair
Jason Laurence Rasgon, Thesis Advisor/Co-Advisor
Elizabeth Ann Mcgraw, Committee Member
Rudolf Johannes Schilder, Committee Member - Keywords:
- Aedes aegypti
Mayaro virus
Relative humidity
Dehydration stress
Vector competence - Abstract:
- The mosquito Aedes aegypti is a competent vector of multiple pathogens including dengue, Zika, yellow fever, chikungunya, and Mayaro viruses. Ae. aegypti is highly invasive and is currently present in the Americas, Oceania, Asia, and Europe, but its distribution and the pathogens it transmits are expected to change due to climate change. Relative humidity is an environmental variable that affects mosquito biology and distribution and can differ between location, habitat, and season, with mosquitoes experiencing significant variation in relative humidity during their lifespan. Low relative humidity can induce dehydration in mosquitoes, leading to alterations in physiological and behavioral responses relevant for pathogen transmission such as bloodfeeding and host-seeking behavior. In this study, we evaluated the short and long-term effects of dehydration stress on mortality and Mayaro virus vector competence in Ae. aegypti. Our results show that exposure to dehydration does not affect viral titers, nor infection, dissemination, and transmission rates, in mosquitoes infected with Mayaro virus. However, we detected a significant effect of dehydration on mosquito mortality and blood-feeding frequency regardless of infection status. The previously observed effects of higher feeding during dehydration and the current observation of altered survival along with no impact on vector competence suggest that the impact of dehydration on viral transmission in mosquitoes will likely be complex.