Development Of A New Nano-scale Vehicle For Delivery Of Curcumin In Biological Systems

Open Access
Author:
Kuo, Chung-fan
Graduate Program:
Bioengineering
Degree:
Master of Science
Document Type:
Master Thesis
Date of Defense:
June 13, 2014
Committee Members:
  • Sheereen Majdzarringhalamaraghy, Thesis Advisor
  • Peter J Butler, Thesis Advisor
  • Mohammad Reza Abidian, Thesis Advisor
Keywords:
  • Lipid-coated PLGA nanoparticles
  • Curcumin
Abstract:
Curcumin, a natural compound extracted from turmeric, has anti-inflammatory, anti-oxidant and neuroprotective properties that make it an attractive therapeutic agent for treatment of disesaes such as cancer and Alzheimer’s disease (AD). Its low water solubility, however, lowers its bioavailability and effectiveness in physiological environment. The goal of this study is to apply nanotechnology to carry curcumin by developing lipid/polymer hybrid nanoparticles which can encapsulate curcumin and improve its bioavailability. To this end, we fabricated and characterized lipid-enveloped poly (D, L-lactide-co-glycolic acid) nanoparticles (PLGA NPs) as a delivery vehicle for curcumin. A modified nanoprecipitation technique was used to fabricate polymeric particles by addressing the injection rate of the polymer solution as one of parameters. Two different approaches were then applied to create lipid monolayer or bilayer coatings on the nanoparticles. We evaluated the effect of non-solvent systems, polymer concentration, injection rate, and polymer/drug ratio on the particle size. After parameter optimization, both lipid bilayer coating and lipid monolayer coating were applied to the curcumin-loaded PLGA nanoparticles. Further examinations including size change, surface charge, colloidal stability, lipid shell stability, encapsulation efficiency, and release profile were conducted to evaluate the physicochemical properties of the lipid-coated curcumin-loaded PLGA nanoparticles.