Filling in the Picture: PNC-1’s Role in Stress Response and Development

Open Access
Lange, Stephanie E
Graduate Program:
Biochemistry and Molecular Biology
Master of Science
Document Type:
Master Thesis
Date of Defense:
March 05, 2014
Committee Members:
  • Wendy Hanna Rose, Thesis Advisor
  • PNC-1
  • C. elegans
  • nicotinamidase
  • sirtuins
  • stress response
Nicotinamide adenine dinucleotide (NAD+) is a vital component of energy metabolism in all organisms. However, this important biomolecule is also consumed by protein modification pathways in times of stress. In order to maintain homeostasis of NAD+ under stress, organisms must utilize de novo synthesis and salvage pathways. In the nematode C. elegans, disruption of the NAD+ salvage results in a variety of detrimental phenotypes. Because of the relationship between this pathway and stress response, I hypothesize that this pathway is regulated in response to stress. Contrary to previous findings in other organisms, I found that this pathway is downregulated in response to oxidative and temperature stress.