Thermal Biosensor Based Micromachined Y-cut Quartz
resonators
Open Access
Author:
Lai, Son
Graduate Program:
Electrical Engineering
Degree:
Master of Science
Document Type:
Master Thesis
Date of Defense:
November 18, 2013
Committee Members:
Srinivas A Tadigadapa, Thesis Advisor/Co-Advisor
Keywords:
Thermal biosensor y-cut quartz
Abstract:
In this thesis, a high-sensitivity, calorimetric, point-of-care device for automated bedside urine testing based on a micromachined thermal sensor is presented. Measurements of the heat of reactions from enzymatic catalysis of glucose, urea and creatinine, were performed using glucose oxidase, urease and creatinine deiminase respectively in liquid batch testing with open air reaction chamber configuration.
For the continuous flow configuration using the integrated fluidic set-up, immobilized urease and creatinine iminohydrolase enzymes on thin (25 μm) Kapton® films are used to measure the catalytic calorimetric output as a function of concentration. The sensor design locates the active Kapton® film in close proximity to the quartz temperature sensor thereby providing an efficient heat coupling between the two. Measurements of urea concentration at room temperature using the continuous flow set-up and creatinine in open chamber setup at 37 °C are presented. Concentrations of 50 mM for urea and 100 μM for creatinine have been measured.
This thesis presents the first non-contact, continuous flow thermal sensing measurements. The unique sensor design allows for enzyme immobilized Kapton® film as an easy to swap disposable cartridge and represents a significant advancement for clinical diagnostic application.
