DEFINED AND XENO-FREE CULTURE FOR HUMAN PLURIPOTENT STEM CELLS

Open Access
Author:
Lin, Tzu Ting
Graduate Program:
Bioengineering
Degree:
Master of Science
Document Type:
Master Thesis
Date of Defense:
March 20, 2017
Committee Members:
  • Xiaojun Lian, Thesis Advisor
  • Justin Brown, Committee Member
  • William Hancock, Committee Member
Keywords:
  • Stem cell culture medium
  • LaSR
  • Xeno-free medium
  • Stem cell culture
  • Human induced pluripotent stem cells
  • Human embryonic stem cells
Abstract:
Owing to their ability to differentiate into all cell types in body, and therefore greatly impact the landscape of regenerative medicine and tissue engineering, the human pluripotent stem cells (hPSCs) including human induced pluripotent stem cells (hiPSCs) and human embryonic stem cells (hESCs) are of great interest to researchers in recent decades. One of the combating issues is the development of a robust stem cell culture environment to maintain stem cells without differentiation for long-term culture, in other words, to support their self-renewal and pluripotency. Moreover, because hiPSCs and hESCs have practical use in in-vivo study, for such a clinical application, it is necessary to establish a chemically defined, feeder-free culture system to maintain large scale undifferentiated stem cells in-vitro. Various methods have been proposed for maintaining large scale stem cell culture Some major considerations are: culture media, extracellular matrix, and environment cues. This study will be focused on developing and analyzing a new xeno-free stem cell culture medium, LaSR, for supporting long-term proliferation and pluripotency for both hiPSCs and hESCs to facilitate the basis of stem cell research.