QUANTITATIVE SURFACE ENHANCED RAMAN SPECTROSCOPY COMBINED
WITH MICROFLUIDICS FOR LAB-ON-A-CHIP TECHNOLOGIES
Open Access
Author:
Koytek, Stephen Michael
Graduate Program:
Engineering Science and Mechanics
Degree:
Master of Science
Document Type:
Master Thesis
Date of Defense:
March 29, 2010
Committee Members:
Melik C Demirel, Thesis Advisor/Co-Advisor Melik C Demirel, Thesis Advisor/Co-Advisor
Keywords:
Raman SERS Nanostructured Nanotechnology PPX Parylene Renal Function disease detection
Abstract:
A highly accurate, real time sensor for biomarker detection is required for early detection of disease. Creatinine concentration in urine can provide important information on the status of the kidney for patients suffering from any disease that affects the kidneys. We prepared nanostructured, surface-enhanced Raman spectroscopy (SERS) substrates without template or lithography that provide controllable, well-organized nanostructures on the surface. This allows for the quantitative analysis of creatinine concentration in urine. Here, we present our work on sensitivity and reliability of the SERS substrate to urine samples collected from diabetic patients and a healthy control group. We report the preparation of a new type of SERS substrate which provides fast (<10s), highly sensitive (creatinine concentration < 0.5 μg/mL) and reproducible (<5% variation) detection of urine. Our method is in good agreement with the contemporary enzymatic method.
