EFFECT OF RETINOID ANALOGS, BOVINE LACTOFERRIN, AND RETINOID RECEPTOR EXPRESSION IN BOVINE MAMMARY CELLS

open_access
Open Access
Author:
Wang, Yinglu
Graduate Program:
Physiology
Degree:
Master of Science
Document Type:
Master Thesis
Date of Defense:
July 17, 2009
Committee Members:
Craig Richard Baumrucker, Thesis Advisor/Co-Advisor
Craig Richard Baumrucker, Thesis Advisor/Co-Advisor
Keywords:
lactoferrin
bovine mammary cells
retinoids
Abstract:
The retinoids are a class of substances with similar chemical structure as retinol. Retinoid signaling has been related to a variety of developmental processes including embryo development, cell cycle arrest and apoptosis, normal growth, vision, reproduction and overall survival. The mechanism of this effect in bovine has not been fully studied. Binding with Retinoic acid receptors (RAR-α, β and γ) or retinoid X receptors (RXR-α, β and γ), retinoids have been shown to induce apoptosis or a G1 arrest and thereby reduce mammary cell number. Our lab was particularly interested in the effect of retinoid signaling in bovine mammary cells. Two cell types, the bovine immortalized (BME-UV1) and primary mammary cells (MeBo), were used as models. Except RXR-γ, all the isotypes of retinoid receptors were expressed in bovine mammary cells. Immortalization of the BME-UV1 cells did not seem to affect retinoid receptor expression, but does affect retinoid effect on cell viability. The effect of natural retinoids (RA), retinoid analogs, and bovine lactoferrin (bLf) on cell viability in vitro was evaluated. We conclude that RAR interaction in bovine mammary cell types regulates cell viability in vitro. In addition, we conclude retinoids and bLf interact with RAR signaling in bovine mammary cells and regulate cell number in vitro, but does not appear to interact with lactogenic hormones.